Mouse SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit
The Mouse SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit is a reliable and efficient tool for the accurate measurement of sirtuin-1 levels in mouse samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing high sensitivity and specificity for precise results.SIRT1 is a key regulator of various cellular processes, including metabolism, longevity, and stress response. Its dysregulation has been linked to a range of diseases, including metabolic disorders, neurodegenerative conditions, and cancer.
Monitoring sirtuin-1 levels can offer valuable insights into these disease mechanisms and aid in the development of therapeutic approaches.Whether investigating novel pathways in cellular biology or exploring potential therapeutic targets, the Mouse SIRT1 ELISA Kit offers a valuable tool for researchers seeking to delve deeper into the intricate world of sirtuin-1 biology. With its reliable performance and ease of use, this kit is a valuable addition to any laboratory conducting mouse studies.
Product Name:
Mouse SIRT1 (NAD-dependent protein deacetylase sirtuin-1) ELISA Kit
SKU:
MOES00911
Target:
Mouse SIRT1 (NAD-dependent protein deacetylase sirtuin-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SIRT1. You can calculate the concentration of Mouse SIRT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
89-102
85-97
Average (%)
93
95
92
1:4
Range (%)
90-106
84-96
85-97
Average (%)
97
90
92
1:8
Range (%)
88-101
84-94
88-102
Average (%)
95
89
94
1:16
Range (%)
89-105
81-95
85-99
Average (%)
96
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
0.9
4.84
0.46
0.81
4.36
Standard deviation
0.02
0.05
0.22
0.03
0.05
0.23
C V (%)
4.17
5.56
4.55
6.52
6.17
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SIRT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SIRT1 in samples. No significant cross-reactivity or interference between Mouse SIRT1 and analogues was observed.
