The Mouse SHH (Hedgehog Homolog Sonic) ELISA Kit is specifically designed for the accurate detection of Sonic Hedgehog levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes. Sonic Hedgehog is a key signaling protein involved in embryonic development, cell differentiation, and tissue regeneration. Dysregulation of the SHH pathway has been linked to various diseases, including cancer, developmental disorders, and neurological conditions.
Therefore, this ELISA kit is an invaluable tool for studying the role of Sonic Hedgehog in these conditions and exploring potential therapeutic interventions. With its user-friendly protocol and reliable performance, the Mouse SHH ELISA Kit from Assay Genie is an essential resource for researchers investigating the intricate mechanisms of Sonic Hedgehog signaling in mouse model systems.
Product Name:
Mouse SHH (Hedgehog Homolog, Sonic) ELISA Kit
SKU:
MOES01131
Target:
Mouse SHH (Hedgehog Homolog, Sonic)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SHH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SHH. You can calculate the concentration of Mouse SHH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
89-104
92-105
Average (%)
101
96
99
1:4
Range (%)
92-107
84-94
88-100
Average (%)
98
89
94
1:8
Range (%)
93-107
81-89
81-95
Average (%)
100
85
87
1:16
Range (%)
88-103
85-96
87-102
Average (%)
95
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.55
111.91
351.15
51.6
116.66
353.01
Standard deviation
2.7
5.58
17.42
2.75
6.87
11.16
C V (%)
5.24
4.99
4.96
5.33
5.89
3.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SHH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SHH in samples. No significant cross-reactivity or interference between Mouse SHH and analogues was observed.
