The Mouse Shh (Hedgehog Homolog Sonic) CLIA Kit is a reliable and accurate tool for detecting levels of Sonic Hedgehog Homolog in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Sonic Hedgehog Homolog is a critical signaling molecule involved in various developmental processes, including embryogenesis and tissue patterning.
Dysregulation of this protein has been linked to several diseases, such as cancer, birth defects, and neurodevelopmental disorders. Therefore, the Mouse Shh CLIA Kit serves as a valuable resource for studying the role of Sonic Hedgehog Homolog in these conditions and exploring potential therapeutic interventions.
Product Name:
Mouse SHH (Hedgehog Homolog, Sonic) CLIA Kit
SKU:
MOES00331
Target:
Mouse SHH (Hedgehog Homolog, Sonic)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SHH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SHH. You can calculate the concentration of Mouse SHH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
88-100
95-106
Average (%)
109
94
100
1:4
Range (%)
88-100
92-104
90-103
Average (%)
95
98
96
1:8
Range (%)
96-114
92-105
93-108
Average (%)
104
99
100
1:16
Range (%)
87-100
89-101
89-99
Average (%)
94
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.19
65.91
197.5
25.9
64.46
196.25
Standard deviation
2.18
7.71
19.65
2.89
4.64
21.76
C V (%)
8.02
11.7
9.95
11.16
7.2
11.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SHH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SHH in samples. No significant cross-reactivity or interference between Mouse SHH and analogues was observed.
