The Mouse SEPP1 (Selenoprotein P) CLIA Kit is a highly sensitive assay designed for the quantitative detection of SEPP1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high specificity and accuracy, ensuring reliable and reproducible results for various research applications.SEPP1, also known as Selenoprotein P, is a crucial protein involved in selenium transport and antioxidant defense. It plays a significant role in regulating selenium homeostasis and protecting cells from oxidative stress, making it a valuable biomarker for studying selenium-related disorders and developing potential therapeutic interventions.
Overall, the Mouse SEPP1 CLIA Kit is a valuable tool for researchers studying the role of SEPP1 in health and disease, offering precise measurements and detailed insights into its functions in mouse models.
Product Name:
Mouse SEPP1 (Selenoprotein P) CLIA Kit
SKU:
MOES00525
Target:
Mouse SEPP1 (Selenoprotein P)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SEPP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SEPP1. You can calculate the concentration of Mouse SEPP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
88-105
98-109
Average (%)
107
96
104
1:4
Range (%)
98-112
85-101
98-111
Average (%)
105
92
104
1:8
Range (%)
89-101
90-105
95-109
Average (%)
96
97
103
1:16
Range (%)
90-104
94-110
102-116
Average (%)
95
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.34
171.66
870.57
104.69
155.76
794.22
Standard deviation
11.76
13.27
60.42
11.08
17.34
48.92
C V (%)
11.06
7.73
6.94
10.58
11.13
6.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SEPP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SEPP1 in samples. No significant cross-reactivity or interference between Mouse SEPP1 and analogues was observed.
