The Mouse SEMA7A (Semaphorin 7A) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of SEMA7A levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.SEMA7A is a protein that plays a crucial role in cell adhesion, migration, and immune response regulation. It is involved in various physiological and pathological processes, including axon guidance, immune responses, and tissue regeneration.
The measurement of SEMA7A levels can provide valuable insights into these processes and help researchers better understand the role of SEMA7A in various diseases and conditions.Overall, the Mouse SEMA7A CLIA Kit is a valuable tool for researchers studying SEMA7A biology and its implications in health and disease. Its high sensitivity and specificity ensure accurate and precise quantification of SEMA7A levels, enabling researchers to advance their knowledge and contribute to the development of potential therapeutic strategies.
Product Name:
Mouse SEMA7A (Semaphorin 7A) CLIA Kit
SKU:
MOES00526
Target:
Mouse SEMA7A (Semaphorin 7A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA7A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SEMA7A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SEMA7A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SEMA7A. You can calculate the concentration of Mouse SEMA7A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
101-115
101-119
Average (%)
102
108
109
1:4
Range (%)
102-116
98-110
96-112
Average (%)
108
104
103
1:8
Range (%)
95-109
98-111
98-114
Average (%)
100
104
104
1:16
Range (%)
94-104
101-117
99-112
Average (%)
99
108
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
105
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.71
385.07
1578.52
198.56
404.79
1626.01
Standard deviation
22.44
36.97
159.9
22.24
36.07
132.52
C V (%)
11.07
9.6
10.13
11.2
8.91
8.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SEMA7A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SEMA7A in samples. No significant cross-reactivity or interference between Mouse SEMA7A and analogues was observed.
