The Mouse Sele (E-Selectin) CLIA Kit is specifically designed for the sensitive and accurate measurement of Sele (E-Selectin) levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Sele (E-Selectin) is a key adhesion molecule involved in the inflammatory response and plays a critical role in leukocyte recruitment to sites of inflammation. Dysregulation of Sele expression has been implicated in various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Mouse Sele (E-Selectin) CLIA Kit, researchers can unravel the intricate mechanisms of inflammatory pathways and explore novel treatment strategies for inflammatory diseases. Trust in the reliability and precision of this kit to advance your research and uncover new insights into the role of Sele in health and disease.
Product Name:
Mouse SELE (E-Selectin) CLIA Kit
SKU:
MOES00249
Target:
Mouse SELE (E-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SELE. You can calculate the concentration of Mouse SELE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
93-108
88-98
Average (%)
91
100
93
1:4
Range (%)
103-117
98-115
90-102
Average (%)
110
106
96
1:8
Range (%)
85-97
85-99
94-104
Average (%)
91
91
99
1:16
Range (%)
92-105
92-107
97-110
Average (%)
98
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.98
128.16
383.97
45.05
119.39
354.84
Standard deviation
4.92
9.01
32.91
5.71
9.91
31.26
C V (%)
10.47
7.03
8.57
12.67
8.3
8.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SELE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SELE in samples. No significant cross-reactivity or interference between Mouse SELE and analogues was observed.
