The Mouse SAA2 (Serum Amyloid A2) CLIA Kit (MOES00614) is a comprehensive assay designed for the precise measurement of SAA2 levels in mouse serum samples. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for various research applications.SAA2, also known as Serum Amyloid A2, is a key protein associated with inflammatory processes and immune response in mice. Elevated SAA2 levels have been linked to conditions such as chronic inflammation, autoimmune disorders, and infectious diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its user-friendly protocol and efficient performance, the Mouse SAA2 CLIA Kit (MOES00614) is an essential tool for researchers seeking to unravel the intricacies of SAA2 biology and its implications in diverse disease states. Upgrade your research capabilities with this innovative kit from Assay Genie.
Product Name:
Mouse SAA2 (Serum Amyloid A2) CLIA Kit
SKU:
MOES00614
Target:
Mouse SAA2 (Serum Amyloid A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SAA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SAA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SAA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SAA2. You can calculate the concentration of Mouse SAA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
90-107
99-115
Average (%)
107
97
108
1:4
Range (%)
85-96
86-97
88-99
Average (%)
91
92
94
1:8
Range (%)
87-102
92-108
103-119
Average (%)
93
100
109
1:16
Range (%)
91-106
93-104
92-103
Average (%)
98
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.45
69.95
399.05
38.09
76.02
428.15
Standard deviation
4.08
7.22
34.44
4.51
6.64
34.94
C V (%)
10.34
10.32
8.63
11.84
8.73
8.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SAA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SAA2 in samples. No significant cross-reactivity or interference between Mouse SAA2 and analogues was observed.
