Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit
The Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit is a reliable and precise tool for quantifying levels of S100B in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable resource for various research endeavors.S100B is a calcium-binding protein that plays a crucial role in various cellular processes, including neuronal function and inflammation. Its dysregulation has been implicated in a range of neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
This ELISA kit enables researchers to explore the role of S100B in disease progression and treatment response, providing valuable insights into its potential as a therapeutic target. With its user-friendly protocol and precise measurements, the Mouse S100B ELISA Kit is a valuable addition to any laboratory conducting research in the field of neuroscience and neurodegenerative diseases.
Product Name:
Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
MOES01455
Target:
Mouse S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100B. You can calculate the concentration of Mouse S100B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
88-101
93-107
Average (%)
99
93
100
1:4
Range (%)
95-110
85-100
91-105
Average (%)
102
92
98
1:8
Range (%)
94-106
87-97
94-106
Average (%)
100
92
99
1:16
Range (%)
94-109
86-96
92-106
Average (%)
101
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
91-106
99
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
269.6
555.8
1940.8
279.5
608.1
1946.9
Standard deviation
13.5
32.8
100.9
19.3
31.6
91.5
C V (%)
5.01
5.9
5.2
6.91
5.2
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse S100B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100B in samples. No significant cross-reactivity or interference between Mouse S100B and analogues was observed.
