Mouse S100A8 (S100 Calcium Binding Protein A8) CLIA Kit
The Mouse S100A8/S100 calcium-binding protein A8 CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of S100A8 levels in mouse biological samples. This kit is ideal for researchers studying inflammation, immune responses, and autoimmune diseases. S100A8, also known as calgranulin A, is a member of the S100 protein family and plays a crucial role in regulating immune responses and inflammation. Elevated levels of S100A8 have been associated with various inflammatory conditions, making it a valuable biomarker for monitoring disease progression and evaluating treatment efficacy.
The Mouse S100A8/S100 calcium-binding protein A8 CLIA Kit offers reliable and reproducible results, making it a valuable tool for investigating the role of S100A8 in mouse models of disease. With its high sensitivity and specificity, this kit is essential for advancing research in the fields of immunology and inflammation.
Product Name:
Mouse S100A8 (S100 Calcium Binding Protein A8) CLIA Kit
SKU:
MOES00608
Target:
Mouse S100A8 (S100 Calcium Binding Protein A8)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100A8. You can calculate the concentration of Mouse S100A8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
85-100
103-115
Average (%)
95
92
108
1:4
Range (%)
96-113
90-101
90-106
Average (%)
104
96
98
1:8
Range (%)
93-106
98-111
94-108
Average (%)
100
105
102
1:16
Range (%)
93-108
85-97
87-100
Average (%)
100
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
423.0
689.84
2927.46
438.01
709.05
3196.54
Standard deviation
44.8
81.95
321.14
43.8
58.64
349.7
C V (%)
10.59
11.88
10.97
10.0
8.27
10.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100A8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
