S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
Product Name:
Mouse S100A4 Recombinant Protein (RPES2525)
Product Code:
RPES2525
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
18A2,42a,Capl,FSp1,metastasin,Mts1,PeL98,pk9a
Accession:
P07091
Sequence:
Ala2-Lys101
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse S100A4 (P07091)(Ala2-Lys101) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
11.7 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
