Mouse S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
The Mouse S100A11 (S100 Calcium Binding Protein A11) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of S100A11 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.S100A11 is a calcium-binding protein that plays a significant role in cell growth, differentiation, and inflammation.
It is implicated in various physiological and pathological processes, making it an important biomarker for studying conditions such as cancer, inflammatory diseases, and neurological disorders. By using this kit, researchers can gain valuable insights into the role of S100A11 in these conditions and potentially develop new therapeutic strategies.
Product Name:
Mouse S100A11 (S100 Calcium Binding Protein A11) CLIA Kit
SKU:
MOES00520
Target:
Mouse S100A11 (S100 Calcium Binding Protein A11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100A11. You can calculate the concentration of Mouse S100A11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-106
95-106
88-98
Average (%)
101
100
93
1:4
Range (%)
89-101
96-110
95-110
Average (%)
95
103
102
1:8
Range (%)
97-111
96-110
103-117
Average (%)
105
103
108
1:16
Range (%)
102-115
93-111
88-101
Average (%)
109
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
385.54
931.49
3433.11
385.83
908.49
3492.04
Standard deviation
33.58
97.06
337.82
36.19
98.21
334.89
C V (%)
8.71
10.42
9.84
9.38
10.81
9.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100A11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A11 in samples. No significant cross-reactivity or interference between Mouse S100A11 and analogues was observed.
