The Mouse REN (Renin) ELISA Kit is specifically designed for the precise measurement of renin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Renin is a key enzyme in the renin-angiotensin system, playing a critical role in regulating blood pressure and electrolyte balance. Dysregulation of renin activity is associated with hypertension, kidney diseases, and other cardiovascular conditions, making it an important target for therapeutic interventions.
By utilizing the Mouse REN ELISA Kit, researchers can effectively study the role of renin in physiological and pathological processes, leading to a better understanding of cardiovascular health and the development of potential treatment strategies.
Product Name:
Mouse REN (Renin) ELISA Kit
SKU:
MOES00673
Target:
Mouse REN (Renin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse REN. You can calculate the concentration of Mouse REN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-112
89-101
Average (%)
92
103
94
1:4
Range (%)
94-109
86-98
97-113
Average (%)
102
91
104
1:8
Range (%)
97-111
85-98
98-112
Average (%)
103
91
104
1:16
Range (%)
97-108
84-95
98-114
Average (%)
102
89
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
92-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
4.3
0.5
1.4
4.7
Standard deviation
0.04
0.06
0.19
0.03
0.06
0.18
C V (%)
8
4.29
4.42
6.0
4.29
3.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse REN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse REN in samples. No significant cross-reactivity or interference between Mouse REN and analogues was observed.
