Mouse REG4 (Regenerating Islet Derived Protein 4) CLIA Kit
The Mouse REG4 (Regenerating Islet-Derived Protein 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of REG4 levels in mouse serum, plasma, and cell culture supernatants. REG4 is a key protein involved in tissue regeneration and inflammatory responses, making it a valuable biomarker for studying conditions such as inflammatory diseases, cancer, and gastrointestinal disorders.
This kit offers reliable and reproducible results, making it suitable for a wide range of research applications. By accurately quantifying REG4 levels, researchers can gain valuable insights into the molecular mechanisms underlying various diseases and potentially discover new therapeutic targets. Upgrade your research with the Mouse REG4 CLIA Kit from Assay Genie.
Product Name:
Mouse REG4 (Regenerating Islet Derived Protein 4) CLIA Kit
SKU:
MOES00516
Target:
Mouse REG4 (Regenerating Islet Derived Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse REG4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REG4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse REG4. You can calculate the concentration of Mouse REG4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
91-105
93-107
Average (%)
104
98
100
1:4
Range (%)
96-111
101-119
84-98
Average (%)
102
108
90
1:8
Range (%)
100-116
95-109
88-102
Average (%)
109
103
94
1:16
Range (%)
103-118
98-116
95-107
Average (%)
109
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.8
95.82
437.65
47.82
88.42
401.85
Standard deviation
5.14
7.98
39.34
4.72
6.63
27.97
C V (%)
10.98
8.33
8.99
9.87
7.5
6.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse REG4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse REG4 in samples. No significant cross-reactivity or interference between Mouse REG4 and analogues was observed.
