The Mouse PYY (Peptide YY) CLIA Kit is a highly reliable and sensitive assay specifically designed for the accurate measurement of PYY levels in mouse serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it an essential tool for research in the fields of metabolism, appetite regulation, and gastrointestinal disorders.PYY is a peptide hormone that plays a crucial role in regulating appetite and energy balance. It is released by the gut in response to food intake, helping to signal satiety and reduce food intake.
Dysregulation of PYY levels has been linked to obesity, diabetes, and other metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.This Mouse PYY CLIA Kit offers a user-friendly protocol, high sensitivity, and a wide detection range, making it suitable for a variety of experimental settings. Researchers can trust in the accuracy and precision of this kit to advance their understanding of PYY biology and its implications in health and disease.
Product Name:
Mouse PYY (Peptide YY) CLIA Kit
SKU:
MOES00620
Target:
Mouse PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PYY. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PYY. You can calculate the concentration of Mouse PYY in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
86-99
101-115
Average (%)
96
91
108
1:4
Range (%)
94-110
91-107
87-99
Average (%)
102
98
92
1:8
Range (%)
93-106
94-107
102-118
Average (%)
98
102
108
1:16
Range (%)
95-108
92-107
85-101
Average (%)
102
98
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.92
72.77
178.01
26.1
68.41
162.41
Standard deviation
3.2
5.41
12.39
2.58
7.55
11.32
C V (%)
11.89
7.43
6.96
9.89
11.04
6.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PYY concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PYY in samples. No significant cross-reactivity or interference between Mouse PYY and analogues was observed.
