The Mouse PTN (Pleiotrophin) CLIA Kit is specifically designed for the precise measurement of pleiotrophin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.Pleiotrophin is a key protein that plays a vital role in various biological processes, including cell growth, differentiation, and tissue repair. Its dysregulation has been linked to numerous diseases, such as cancer and neurological disorders, making it a valuable biomarker for disease research and therapeutic development.
With its advanced technology and reliable performance, the Mouse PTN (Pleiotrophin) CLIA Kit is an indispensable tool for researchers studying the role of pleiotrophin in health and disease. Visit our website for more information: www.assaygenie.com/mouse-ptn-pleiotrophin-clia-kit-moes00490/
Product Name:
Mouse PTN (Pleiotrophin) CLIA Kit
SKU:
MOES00490
Target:
Mouse PTN (Pleiotrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PTN. You can calculate the concentration of Mouse PTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
85-98
101-111
Average (%)
93
90
106
1:4
Range (%)
91-107
99-114
101-114
Average (%)
98
107
107
1:8
Range (%)
97-112
97-109
100-117
Average (%)
103
102
107
1:16
Range (%)
102-118
93-108
85-97
Average (%)
109
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.42
100.97
345.49
39.34
95.44
319.16
Standard deviation
4.17
11.69
39.63
4.17
11.09
30.13
C V (%)
11.14
11.58
11.47
10.6
11.62
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PTN in samples. No significant cross-reactivity or interference between Mouse PTN and analogues was observed.
