The Mouse PRF1 (Perforin-1) ELISA Kit is specifically designed for the accurate measurement of perforin-1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an invaluable tool for a variety of research applications.Perforin-1 is a key protein involved in the immune response, specifically in the cytotoxic activity of T and NK cells.
It plays a crucial role in eliminating virally infected and transformed cells, highlighting its importance in immune surveillance and defense mechanisms. The measurement of perforin-1 levels can provide valuable insights into immune function and responses, making this ELISA kit essential for studying immune-related diseases and therapies.
Product Name:
Mouse PRF1 (Perforin 1) ELISA Kit
SKU:
MOES01347
Target:
Mouse PRF1 (Perforin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PRF1. You can calculate the concentration of Mouse PRF1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
86-100
91-104
Average (%)
93
92
98
1:4
Range (%)
88-101
86-99
84-96
Average (%)
96
91
90
1:8
Range (%)
85-98
81-93
88-101
Average (%)
92
88
94
1:16
Range (%)
92-106
86-98
82-95
Average (%)
100
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.22
4.57
0.49
1.17
4.45
Standard deviation
0.03
0.07
0.22
0.03
0.05
0.21
C V (%)
6.25
5.74
4.81
6.12
4.27
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PRF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PRF1 in samples. No significant cross-reactivity or interference between Mouse PRF1 and analogues was observed.
