Mouse pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
The Mouse pMAPT (phosphorylated microtubule-associated protein tau) ELISA Kit is specifically designed for the precise measurement of phosphorylated microtubule-associated protein tau levels in mouse serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research applications.Phosphorylated microtubule-associated protein tau is a key protein involved in neurodegenerative disorders such as Alzheimer's disease, playing a critical role in the formation of neurofibrillary tangles and neuronal dysfunction.
By accurately measuring pMAPT levels, researchers can gain valuable insights into the pathology of these disorders and potentially develop novel therapeutic strategies.With the Mouse pMAPT ELISA Kit, researchers can confidently study the role of phosphorylated microtubule-associated protein tau in neurodegenerative diseases and advance their understanding of these complex conditions. Trust in the reliability and precision of this kit to drive groundbreaking discoveries in the field of neuroscience.
Product Name:
Mouse pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
SKU:
MOES01646
Target:
Mouse pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse pMAPT/pTAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse pMAPT/pTAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse pMAPT/pTAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse pMAPT/pTAU. You can calculate the concentration of Mouse pMAPT/pTAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
99-115
87-101
Average (%)
92
105
93
1:4
Range (%)
94-107
86-98
84-97
Average (%)
99
91
89
1:8
Range (%)
88-102
83-95
86-100
Average (%)
94
90
92
1:16
Range (%)
88-104
78-90
85-96
Average (%)
95
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.26
141.34
345.67
52.1
145.27
334.9
Standard deviation
2.66
6.2
14.9
2.93
7.71
16.34
C V (%)
5.29
4.39
4.31
5.62
5.31
4.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse pMAPT/pTAU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse pMAPT/pTAU in samples. No significant cross-reactivity or interference between Mouse pMAPT/pTAU and analogues was observed.
