Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit
The Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit is a powerful tool for the quantification of PIIINP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering reliable and reproducible results for a variety of research applications.PIIINP is a critical marker of collagen synthesis and turnover, serving as a valuable indicator of fibrosis and tissue remodeling processes. By accurately measuring PIIINP levels, researchers can gain insight into various pathological conditions, including liver fibrosis, pulmonary fibrosis, and other connective tissue disorders.
With its ease of use and robust performance, the Mouse PIIINP ELISA Kit is an essential asset for studies investigating collagen metabolism and fibrotic diseases in mouse models. Trust in this kit to provide accurate and precise measurements, advancing your research efforts in the field of fibrosis and tissue remodeling.
Product Name:
Mouse PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit
SKU:
MOES01716
Target:
Mouse PIIINP (N-Terminal Procollagen III Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅢNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PⅢNP. You can calculate the concentration of Mouse PⅢNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
89-102
95-110
Average (%)
98
94
103
1:4
Range (%)
88-103
80-91
89-101
Average (%)
95
86
94
1:8
Range (%)
92-107
85-98
89-101
Average (%)
99
92
94
1:16
Range (%)
92-107
80-91
84-94
Average (%)
98
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.11
4.38
0.47
1.05
4.61
Standard deviation
0.03
0.07
0.23
0.03
0.04
0.22
C V (%)
6
6.31
5.25
6.38
3.81
4.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PⅢNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PⅢNP in samples. No significant cross-reactivity or interference between Mouse PⅢNP and analogues was observed.
