Mouse PICP (Procollagen I C-terminal Propeptide) ELISA Kit
The Mouse PICP (Procollagen I C-Terminal Propeptide) ELISA Kit is specifically designed for the sensitive and accurate measurement of PICP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring dependable and reproducible results for a variety of research applications.PICP is a key marker for collagen synthesis and is essential for the formation of collagen type I, the most abundant collagen in the body. Monitoring PICP levels can provide valuable insights into collagen metabolism, tissue remodeling, and various disease states such as fibrosis, osteoporosis, and connective tissue disorders.
With its easy-to-use format and reliable performance, the Mouse PICP ELISA Kit is a valuable tool for researchers studying collagen biology, extracellular matrix dynamics, and pathologies related to collagen abnormalities. Order now to streamline your experiments and advance your research in this important field.
Product Name:
Mouse PICP (Procollagen I C-terminal Propeptide) ELISA Kit
SKU:
MOES00804
Target:
Mouse PICP (Procollagen I C-terminal Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PⅠCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PⅠCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PⅠCP. You can calculate the concentration of Mouse PⅠCP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
93-105
97-110
Average (%)
98
99
103
1:4
Range (%)
86-98
83-97
87-98
Average (%)
93
89
92
1:8
Range (%)
86-101
87-98
88-100
Average (%)
92
93
94
1:16
Range (%)
90-105
83-97
84-98
Average (%)
96
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.26
321.15
863.49
94.71
313.21
836.71
Standard deviation
6.69
13.87
41.36
6.0
15.72
38.4
C V (%)
6.48
4.32
4.79
6.34
5.02
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PⅠCP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PⅠCP in samples. No significant cross-reactivity or interference between Mouse PⅠCP and analogues was observed.
