The Mouse Proinsulin ELISA Kit is specifically designed for the precise measurement of proinsulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Proinsulin is a key precursor to insulin, playing a crucial role in glucose metabolism and regulation of blood sugar levels.
Abnormal proinsulin levels have been linked to diabetes and other metabolic disorders, highlighting the importance of studying this biomarker in disease research and potential therapeutic development.With easy-to-follow protocols and quick assay time, the Mouse Proinsulin ELISA Kit from Assaygenie is a valuable tool for investigating proinsulin biology and its implications in health and disease.
Product Name:
Mouse PI (Proinsulin) ELISA Kit
SKU:
MOES01390
Target:
Mouse PI (Proinsulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PI. You can calculate the concentration of Mouse PI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
97-112
94-107
Average (%)
101
104
99
1:4
Range (%)
96-109
82-95
96-110
Average (%)
101
88
104
1:8
Range (%)
99-114
84-96
92-106
Average (%)
105
91
100
1:16
Range (%)
98-113
80-92
96-113
Average (%)
106
86
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.9
3.6
0.5
0.9
3.5
Standard deviation
0.04
0.04
0.12
0.03
0.05
0.12
C V (%)
8
4.44
3.33
6.0
5.56
3.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PI in samples. No significant cross-reactivity or interference between Mouse PI and analogues was observed.
