The Mouse PGF (Placental Growth Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of PGF levels in mouse serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PGF is a key growth factor involved in angiogenesis and vascular development, playing a critical role in various physiological and pathological processes.
It is essential for normal placental development and function, making it a valuable biomarker for studying pregnancy-related complications and disorders.With its high sensitivity and specificity, the Mouse PGF CLIA Kit is a valuable tool for researchers studying angiogenesis, vascular development, and reproductive health in mouse models. Get accurate and reliable results for your research with this innovative kit from Assay Genie.
Product Name:
Mouse PGF (Placental Growth Factor) CLIA Kit
SKU:
MOES00477
Target:
Mouse PGF (Placental Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PGF. You can calculate the concentration of Mouse PGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
95-110
88-104
Average (%)
96
102
95
1:4
Range (%)
90-104
95-110
102-115
Average (%)
97
102
108
1:8
Range (%)
93-106
90-103
91-105
Average (%)
100
96
96
1:16
Range (%)
97-111
102-115
99-116
Average (%)
102
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
100-113
107
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.19
49.05
141.93
18.85
47.07
140.68
Standard deviation
1.85
4.17
10.59
2.18
4.68
12.9
C V (%)
9.64
8.5
7.46
11.56
9.94
9.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PGF in samples. No significant cross-reactivity or interference between Mouse PGF and analogues was observed.
