The Mouse PEDF (Pigment Epithelium-Derived Factor) CLIA Kit is a powerful tool for measuring PEDF levels in mouse serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit offers accurate and dependable results for a variety of research applications.PEDF is a multifunctional protein known for its anti-angiogenic and neurotrophic properties. It plays a critical role in regulating processes such as angiogenesis, inflammation, and cell survival, making it a valuable biomarker for studying various diseases and potential therapeutic interventions.
This kit provides researchers with a reliable method for quantifying PEDF levels in mouse samples, enabling a better understanding of the role of this protein in physiological and pathological conditions. Trust the Mouse PEDF CLIA Kit for precise and consistent results in your research endeavors.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PEDF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PEDF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PEDF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PEDF. You can calculate the concentration of Mouse PEDF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
85-96
95-110
Average (%)
107
91
101
1:4
Range (%)
90-104
89-102
97-114
Average (%)
96
96
105
1:8
Range (%)
94-106
99-113
93-103
Average (%)
99
105
98
1:16
Range (%)
91-105
91-106
90-106
Average (%)
98
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.79
259.44
731.53
114.16
248.06
674.58
Standard deviation
12.97
26.72
55.3
14.61
23.69
53.29
C V (%)
12.03
10.3
7.56
12.8
9.55
7.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PEDF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PEDF in samples. No significant cross-reactivity or interference between Mouse PEDF and analogues was observed.
