Mouse PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit
The Mouse PDGFB (Platelet-Derived Growth Factor Subunit B) CLIA Kit is a highly efficient and reliable assay kit designed for the accurate measurement of PDGFB levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides precise and reproducible results, making it an invaluable tool for a wide range of research applications.PDGFB is a key growth factor that plays a crucial role in regulating cell growth, differentiation, and tissue repair. It is involved in various biological processes, such as angiogenesis, wound healing, and fibrosis, making it a valuable target for studying diseases like cancer, cardiovascular disorders, and fibrotic conditions.
By measuring PDGFB levels, researchers can gain insights into the pathogenesis of these diseases and potentially develop targeted therapies.Overall, the Mouse PDGFB CLIA Kit offers researchers a powerful tool to study the role of PDGFB in various physiological and pathological processes, providing valuable information for advancing our understanding of disease mechanisms and developing novel therapeutic strategies.
Product Name:
Mouse PDGFB (Platelet Derived Growth Factor Subunit B) CLIA Kit
SKU:
MOES00486
Target:
Mouse PDGFB (Platelet Derived Growth Factor Subunit B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PDGFB. You can calculate the concentration of Mouse PDGFB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
93-104
86-97
Average (%)
99
98
92
1:4
Range (%)
96-112
101-116
86-98
Average (%)
103
109
92
1:8
Range (%)
83-98
90-106
88-100
Average (%)
90
97
93
1:16
Range (%)
99-114
85-101
91-105
Average (%)
105
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
94
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.59
67.3
382.3
37.1
72.31
404.01
Standard deviation
3.88
5.53
40.75
3.87
8.02
26.71
C V (%)
9.56
8.22
10.66
10.43
11.09
6.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PDGFB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGFB in samples. No significant cross-reactivity or interference between Mouse PDGFB and analogues was observed.
