The Mouse PDGF-BB (Platelet-Derived Growth Factor-BB) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PDGF-BB levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.PDGF-BB is a key growth factor involved in cell growth, proliferation, and wound healing, making it essential for various biological processes.
Dysregulation of PDGF-BB has been implicated in diseases such as cancer and cardiovascular disorders, highlighting its importance as a biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse PDGF-BB CLIA Kit offers researchers a valuable tool for investigating the role of PDGF-BB in health and disease, providing valuable insights for potential therapeutic strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGF-BB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PDGF-BB. You can calculate the concentration of Mouse PDGF-BB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
103-119
94-110
Average (%)
105
110
101
1:4
Range (%)
104-120
100-116
94-109
Average (%)
110
106
101
1:8
Range (%)
90-104
88-103
90-104
Average (%)
97
95
95
1:16
Range (%)
99-113
89-101
96-110
Average (%)
107
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.8
122.55
423.67
45.57
113.89
423.93
Standard deviation
4.19
12.52
34.27
4.7
13.46
47.69
C V (%)
9.15
10.22
8.09
10.31
11.82
11.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PDGF-BB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGF-BB in samples. No significant cross-reactivity or interference between Mouse PDGF-BB and analogues was observed.
