The Mouse PCX (Podocalyxin) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of podocalyxin levels in mouse samples. Podocalyxin is a key protein involved in various physiological and pathological processes, including cell adhesion, migration, and kidney function.This kit offers reliable and reproducible results, making it suitable for use in a wide range of research applications, including studies on kidney diseases, cancer, and inflammation.
By accurately measuring podocalyxin levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Mouse PCX CLIA Kit is a valuable tool for researchers seeking to deepen their understanding of podocalyxin biology and its role in health and disease in mouse models.
Product Name:
Mouse PCX (Podocalyxin) CLIA Kit
SKU:
MOES00491
Target:
Mouse PCX (Podocalyxin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PCX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PCX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PCX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PCX. You can calculate the concentration of Mouse PCX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-101
91-105
104-119
Average (%)
96
96
110
1:4
Range (%)
94-108
88-99
101-117
Average (%)
101
94
108
1:8
Range (%)
91-104
86-97
103-119
Average (%)
97
91
109
1:16
Range (%)
95-112
86-100
85-99
Average (%)
102
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.09
189.8
996.9
107.37
189.4
1027.29
Standard deviation
10.62
21.58
68.89
12.17
15.64
74.58
C V (%)
10.11
11.37
6.91
11.33
8.26
7.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PCX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PCX in samples. No significant cross-reactivity or interference between Mouse PCX and analogues was observed.
