Mouse PAPPA (Pregnancy Associated Plasma Protein A) CLIA Kit
The Mouse PAPP-A (Pregnancy-associated plasma protein A) CLIA Kit is specifically designed for the accurate measurement of PAPP-A levels in mouse serum, plasma, and tissue lysate samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Mouse PAPP-A is an important protein involved in pregnancy and fetal development, as well as in various physiological processes such as insulin-like growth factor regulation and tissue remodeling.
It serves as a key biomarker in studies related to pregnancy, fertility, and development, making this kit an essential tool for researchers investigating these areas.For more information and to purchase the Mouse PAPP-A CLIA Kit, please visit the link provided: www.assaygenie.com/mouse-pappa-pregnancy-associated-plasma-protein-a-clia-kit-moes00067/
Product Name:
Mouse PAPPA (Pregnancy Associated Plasma Protein A) CLIA Kit
SKU:
MOES00067
Target:
Mouse PAPPA (Pregnancy Associated Plasma Protein A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAPPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAPPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PAPPA. You can calculate the concentration of Mouse PAPPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
101-112
102-114
Average (%)
96
106
108
1:4
Range (%)
99-112
87-100
87-103
Average (%)
106
93
94
1:8
Range (%)
103-116
98-111
90-104
Average (%)
109
105
95
1:16
Range (%)
96-111
103-116
100-118
Average (%)
104
110
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
105
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.77
254.44
747.73
97.92
252.88
736.47
Standard deviation
8.45
23.31
85.47
9.59
27.34
57.74
C V (%)
8.73
9.16
11.43
9.79
10.81
7.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PAPPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PAPPA in samples. No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed.
