Mouse P4HB Recombinant Protein (RPPB2065)
- SKU:
- RPPB2065
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- P4HB
- Synonyms:
- Protein disulfide-isomerase
- PDI
- Cellular thyroidhormone-binding protein
- Endoplasmic reticulum resident protein 59
- Source:
- Sf9 Insect cells
- Uniprot:
- P09103
Description
| Product Name: | Mouse P4HB Recombinant Protein |
| Product Code: | RPPB2065 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | P4HB |
| Synonyms: | Protein disulfide-isomerase, PDI, Cellular thyroidhormone-binding protein, Endoplasmic reticulum resident protein 59, ER protein59, ERp59, Prolyl 4-hydroxylase subunit beta, p55. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | P4HB protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAAAKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKN GDTASPKEYT AGREADDIVN WLKKRTGPAATTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQLDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPKIFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRLITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANFEEVAFDEKKN VFVEFYAPWCGHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDGAGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH |
P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
P4HB produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 498 amino acids (20-509a.a.) andhaving a molecular mass of 56.1kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).P4HB is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family. |
| UniProt Protein Details: | Protein type:Isomerase; Nuclear receptor co-regulator; EC 5.3.4.1; Endoplasmic reticulum; Oxidoreductase Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane; ER-Golgi intermediate compartment Molecular Function:enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; protein disulfide isomerase activity; isomerase activity; peptidyl-proline 4-dioxygenase activity Biological Process: protein folding; cell redox homeostasis; peptidyl-proline hydroxylation to 4-hydroxy-L-proline |
| UniProt Code: | P09103 |
| NCBI GenInfo Identifier: | 42415475 |
| NCBI Gene ID: | 18453 |
| NCBI Accession: | NP_035162.1 |
| UniProt Secondary Accession: | P09103,Q922C8, |
| UniProt Related Accession: | P09103 |
| Molecular Weight: | 60.8kDa |
| NCBI Full Name: | protein disulfide-isomerase |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase, beta polypeptide |
| NCBI Official Symbol: | P4hb |
| NCBI Official Synonym Symbols: | PDI; Thbp; ERp59; Pdia1 |
| NCBI Protein Information: | protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59 |
| UniProt Protein Name: | Protein disulfide-isomerase |
| UniProt Synonym Protein Names: | Cellular thyroid hormone-binding protein; Endoplasmic reticulum resident protein 59; ER protein 59; ERp59; Prolyl 4-hydroxylase subunit beta; p55 |
| UniProt Gene Name: | P4hb |
| UniProt Entry Name: | PDIA1_MOUSE |
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