The Mouse OxLDL (Oxidized Low-Density Lipoprotein) CLIA Kit is specifically designed for the accurate and sensitive detection of oxidized LDL levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring reliable results for various research applications.Oxidized LDL is a key marker of oxidative stress and plays a critical role in the development of atherosclerosis and cardiovascular diseases.
Monitoring OxLDL levels can provide valuable insights into the progression of these conditions and support the development of targeted therapies.With its high sensitivity and easy-to-use format, the Mouse OxLDL CLIA Kit is an essential tool for researchers studying oxidative stress, cardiovascular health, and other related fields. Get reliable and accurate results with this comprehensive kit.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OxLDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OxLDL. You can calculate the concentration of Mouse OxLDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
89-102
93-109
Average (%)
93
95
99
1:4
Range (%)
98-109
93-106
97-113
Average (%)
103
99
104
1:8
Range (%)
87-101
87-99
92-109
Average (%)
92
93
100
1:16
Range (%)
99-113
97-111
101-117
Average (%)
106
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
88-105
96
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
207.12
602.33
1455.6
191.32
567.69
1422.8
Standard deviation
23.43
53.61
149.2
20.82
47.91
111.12
C V (%)
11.31
8.9
10.25
10.88
8.44
7.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OxLDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OxLDL in samples. No significant cross-reactivity or interference between Mouse OxLDL and analogues was observed.
