The Mouse OPN (Osteopontin) CLIA Kit is specifically designed for the quantitative detection of osteopontin levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results for a variety of research applications.Osteopontin is a key protein involved in bone metabolism and immune regulation, playing a critical role in various diseases such as osteoporosis, cancer, and inflammatory disorders.
By measuring osteopontin levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify new therapeutic targets.Overall, the Mouse OPN CLIA Kit from Assay Genie is a valuable tool for studying the role of osteopontin in health and disease, offering precise and reliable measurements to advance your research efforts.
Product Name:
Mouse OPN (Osteopontin) CLIA Kit
SKU:
MOES00335
Target:
Mouse OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OPN. You can calculate the concentration of Mouse OPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
91-106
87-102
Average (%)
102
97
94
1:4
Range (%)
86-97
95-108
85-97
Average (%)
91
100
91
1:8
Range (%)
85-99
85-99
87-102
Average (%)
92
91
94
1:16
Range (%)
88-102
101-119
85-99
Average (%)
95
109
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
92-105
100
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.55
123.38
472.15
54.73
112.6
445.35
Standard deviation
5.13
12.81
45.0
6.21
9.18
48.1
C V (%)
9.4
10.38
9.53
11.35
8.15
10.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OPN in samples. No significant cross-reactivity or interference between Mouse OPN and analogues was observed.
