The Mouse OPG (Osteoprotegerin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of OPG levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications in the field of osteoporosis and bone health.OPG is a key regulator of bone remodeling, acting as a decoy receptor for RANKL and inhibiting osteoclast formation and activity. Dysregulation of the OPG/RANKL signaling pathway is implicated in various bone-related diseases, such as osteoporosis and arthritis, making OPG a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its high sensitivity and specificity, the Mouse OPG CLIA Kit offers researchers a reliable and efficient means of quantifying OPG levels in mouse samples, enabling detailed investigations into the role of OPG in skeletal biology and the development of novel treatments for bone disorders.
Product Name:
Mouse OPG (Osteoprotegerin) CLIA Kit
SKU:
MOES00062
Target:
Mouse OPG (Osteoprotegerin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OPG. You can calculate the concentration of Mouse OPG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
96-110
89-100
Average (%)
106
103
94
1:4
Range (%)
100-117
99-115
102-116
Average (%)
107
107
109
1:8
Range (%)
101-117
90-106
95-111
Average (%)
110
97
101
1:16
Range (%)
87-102
103-117
87-98
Average (%)
93
110
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
105
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.32
132.49
393.44
50.6
120.87
389.44
Standard deviation
5.5
13.92
24.12
4.82
12.24
30.92
C V (%)
10.93
10.51
6.13
9.53
10.13
7.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed.
