The Mouse NUP160 (Nucleoporin 160kDa) CLIA Kit is a cutting-edge assay designed for the quantitative measurement of NUP160 levels in mouse samples. This kit offers superior sensitivity and specificity, allowing for precise and accurate results to be obtained for research purposes.NUP160 is a key component of the nuclear pore complex, playing a crucial role in regulating nucleocytoplasmic transport and maintaining cellular homeostasis. Dysfunction of NUP160 has been linked to various diseases, including neurological disorders and cancer, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its user-friendly protocol and reliable performance, the Mouse NUP160 CLIA Kit is the perfect tool for researchers looking to investigate the role of NUP160 in health and disease. Order yours today and unlock new insights into cellular function and pathogenesis.
Product Name:
Mouse NUP160 (Nucleoporin 160kDa) CLIA Kit
SKU:
MOES00458
Target:
Mouse NUP160 (Nucleoporin 160kDa)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NUP160. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NUP160 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NUP160, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NUP160. You can calculate the concentration of Mouse NUP160 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
88-100
88-102
Average (%)
98
95
95
1:4
Range (%)
92-109
98-112
91-104
Average (%)
100
106
97
1:8
Range (%)
91-102
97-110
90-105
Average (%)
96
103
96
1:16
Range (%)
104-120
87-99
89-102
Average (%)
110
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.84
300.42
734.69
113.31
324.44
719.84
Standard deviation
9.96
21.12
67.74
11.69
23.62
43.91
C V (%)
8.99
7.03
9.22
10.32
7.28
6.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NUP160 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NUP160 in samples. No significant cross-reactivity or interference between Mouse NUP160 and analogues was observed.
