The Mouse NT-3 (Neurotrophin 3) CLIA Kit is a powerful tool for the quantitative measurement of NT-3 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, providing researchers with reliable and accurate results for a variety of experimental applications.Neurotrophin 3 is a key neurotrophic factor that plays a critical role in the development and maintenance of the nervous system. It is involved in neuronal survival, differentiation, and synaptic plasticity, making it a valuable target for studying conditions such as neurodegenerative diseases, spinal cord injuries, and cognitive disorders.
By utilizing the Mouse NT-3 CLIA Kit, researchers can gain valuable insights into NT-3 levels in mouse models, helping to further our understanding of the role of this important neurotrophin in health and disease. This kit is an essential tool for any laboratory conducting research in the field of neuroscience and neurobiology.
Product Name:
Mouse NT-3 (Neurotrophin 3) CLIA Kit
SKU:
MOES00450
Target:
Mouse NT-3 (Neurotrophin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NT-3. You can calculate the concentration of Mouse NT-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-114
87-98
96-109
Average (%)
109
92
102
1:4
Range (%)
101-116
87-98
85-100
Average (%)
107
92
92
1:8
Range (%)
103-117
85-101
86-100
Average (%)
109
92
91
1:16
Range (%)
95-112
87-100
98-111
Average (%)
103
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.22
224.93
911.01
102.33
246.41
978.32
Standard deviation
10.8
16.04
104.4
11.06
26.54
61.63
C V (%)
11.34
7.13
11.46
10.81
10.77
6.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NT-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NT-3 in samples. No significant cross-reactivity or interference between Mouse NT-3 and analogues was observed.
