The Mouse NRF2 (Nuclear Factor Erythroid 2-Related Factor 2) ELISA Kit is a reliable tool for detecting NRF2 levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal choice for a variety of research purposes.NRF2 is a key transcription factor that plays a crucial role in regulating the antioxidant response element (ARE) pathway, which is essential for protecting cells from oxidative stress and inflammation.
Dysregulation of NRF2 has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic disorders.By measuring NRF2 levels using this ELISA kit, researchers can gain valuable insights into the role of NRF2 in disease pathogenesis and explore potential therapeutic strategies targeting NRF2. Upgrade your research with the Mouse NRF2 ELISA Kit from Assay Genie.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NFE2L2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NFE2L2. You can calculate the concentration of Mouse NFE2L2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
85-98
94-110
Average (%)
94
91
100
1:4
Range (%)
91-108
81-94
89-103
Average (%)
99
87
94
1:8
Range (%)
86-100
79-93
81-93
Average (%)
93
86
88
1:16
Range (%)
88-100
83-97
84-98
Average (%)
93
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.0
7.44
1.2
2.05
6.84
Standard deviation
0.06
0.09
0.4
0.07
0.11
0.34
C V (%)
5.5
4.5
5.38
5.83
5.37
4.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NFE2L2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NFE2L2 in samples. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.
