The Mouse NPHN (Nephrin) ELISA Kit is specifically designed for the accurate quantification of nephrin levels in mouse serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides researchers with reliable and reproducible results, making it a valuable tool for various research applications. Nephrin is a critical protein involved in the maintenance of the kidney filtration barrier, playing a crucial role in kidney function and overall health. Dysregulation of nephrin expression has been implicated in various kidney diseases, including nephrotic syndrome and diabetic nephropathy, making it a key biomarker for studying these conditions and potential therapeutic interventions.
This ELISA kit offers a simple and efficient method for measuring mouse nephrin levels, providing researchers with valuable insights into kidney health and disease progression. It is an essential tool for those studying kidney disorders and seeking to better understand the role of nephrin in maintaining kidney function.
Product Name:
Mouse NPHN (Nephrin) ELISA Kit
SKU:
MOES01693
Target:
Mouse NPHN (Nephrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NPHN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NPHN. You can calculate the concentration of Mouse NPHN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
95-108
91-106
Average (%)
92
102
97
1:4
Range (%)
90-105
82-94
87-100
Average (%)
98
89
93
1:8
Range (%)
93-108
86-99
86-99
Average (%)
99
91
91
1:16
Range (%)
86-100
80-90
86-101
Average (%)
93
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.0
4.89
0.49
0.97
4.86
Standard deviation
0.03
0.04
0.15
0.03
0.06
0.22
C V (%)
5.77
4.0
3.07
6.12
6.19
4.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NPHN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NPHN in samples. No significant cross-reactivity or interference between Mouse NPHN and analogues was observed.
