The Mouse NOS2 (iNOS) Nitric Oxide Synthase 2 Inducible ELISA Kit is specially designed for the accurate and reliable detection of inducible nitric oxide synthase 2 (iNOS) levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.iNOS is an enzyme that plays a critical role in the production of nitric oxide, a key signaling molecule involved in various physiological and pathological processes.
Dysregulation of iNOS activity has been linked to inflammatory disorders, autoimmune diseases, and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse NOS2 (iNOS) Nitric Oxide Synthase 2 Inducible ELISA Kit is an essential tool for researchers seeking to investigate the role of iNOS in mouse models and better understand its implications in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
95-109
94-106
Average (%)
91
103
99
1:4
Range (%)
94-110
87-100
93-109
Average (%)
102
94
100
1:8
Range (%)
94-109
87-101
97-110
Average (%)
100
93
103
1:16
Range (%)
96-108
81-94
94-108
Average (%)
103
87
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.9
9.9
1.0
3.0
10.5
Standard deviation
0.1
0.2
0.3
0.1
0.2
0.5
C V (%)
9.09
6.9
3.03
10.0
6.67
4.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
