Mouse NKp46 Recombinant Protein (RPPB4102)
- SKU:
- RPPB4102
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Mouse
- Target:
- NKp46
- Synonyms:
- Natural cytotoxicity triggering receptor 1
- Activating receptor 1
- mAR-1
- Lymphocyte antigen 94
- Source:
- Sf9 Insect cells
- Uniprot:
- Q8C567
Description
| Product Name: | Mouse NKp46 Recombinant Protein |
| Product Code: | RPPB4102 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | NKp46 |
| Synonyms: | Natural cytotoxicity triggering receptor 1, Activating receptor 1, mAR-1, Lymphocyte antigen 94, Natural killer cell p46-related protein, NK-p46, NKp46, mNKp46, CD335, Ncr1, Ly94. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | NKp46 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADLQRINTEK ETLPKPIIWA KPSIMVTNGN SVNIWCQGAQ SASEYQLYFE GSFFALERPK PSRSMNKVRF FISQMTSHTA GIYTCFYQSG ELWSKSSNPL KLVVTGLYDT PNLWVYPRPE VTLGENVTFF CQLKTATSKF FLLKERGSNH IQNKYGNIQA EFPMGPVTRA HRGTYRCFGSYNDYAWSFPS EPVTLLITGG VENSSLAPTD PTSSLDYWEF DLSTNESGLQ KDSAFWDHTT QNLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPIEKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H |
A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30, which are expressed on circulating NKcells, and NKp44, which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. The Lysis of tumor cells by NK-cells involves recognition by NKp46 of heparan sulfate moieties of membrane heparan sulfate proteoglycans. Furthermore, NKp46 is a surface receptor involved in NK-cell cell death by apoptosis. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46 (22-255aa) is purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. In addition, engagement of the antigen with the monoclonal antibody stimulates intracellular calcium levels and the synthesis of cytokines. CD59 is an NKp46 coreceptor (by physical association) together they activate cytotoxicity of human NK-cells, their engagement results in tyrosine phosphorylation of CD3-zeta chains associated with NKp46. Reduced cell surface expression of NKp46 and other NK-cell receptors is linked to the impaired NK-cell cytolytic function in viremic HIV-1 infection.
NKp46 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 481 amino acids (17-255 a.a) and having a molecular mass of 54.4kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).NKp46 is fused to a 239 amino acid hIgG-His Tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NCR1: Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Belongs to the natural cytotoxicity receptor (NCR) family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 7|7 A1 Cellular Component: integral component of membrane; membrane; plasma membrane Biological Process: defense response to virus; detection of virus |
| UniProt Code: | Q8C567 |
| NCBI GenInfo Identifier: | 31981638 |
| NCBI Gene ID: | 17086 |
| NCBI Accession: | NP_034876.2 |
| UniProt Secondary Accession: | Q8C567,Q1RLN4, Q80UY6, Q9Z0Q4, |
| UniProt Related Accession: | Q8C567 |
| Molecular Weight: | |
| NCBI Full Name: | natural cytotoxicity triggering receptor 1 |
| NCBI Synonym Full Names: | natural cytotoxicity triggering receptor 1 |
| NCBI Official Symbol: | Ncr1 |
| NCBI Official Synonym Symbols: | Ly94; NKp46 |
| NCBI Protein Information: | natural cytotoxicity triggering receptor 1 |
| UniProt Protein Name: | Natural cytotoxicity triggering receptor 1 |
| UniProt Synonym Protein Names: | Activating receptor 1; mAR-1; Lymphocyte antigen 94; Natural killer cell p46-related protein; NK-p46; NKp46; mNKp46; CD_antigen: CD335 |
| Protein Family: | Niemann-Pick type C-related protein |
| UniProt Gene Name: | Ncr1 |
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