The Mouse NGF (Nerve Growth Factor) ELISA Kit is specifically designed for the precise measurement of NGF levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research purposes.NGF is a key protein that plays a vital role in promoting the growth and survival of nerve cells. It is essential for the development and maintenance of the nervous system, making it a crucial factor in neurobiology research.
Additionally, NGF has been implicated in conditions such as Alzheimer's disease and peripheral neuropathy, underscoring its importance as a biomarker for these disorders.Overall, the Mouse NGF ELISA kit is a valuable tool for scientists studying neurotrophic factors and their role in neurodegenerative diseases, offering reliable and reproducible results for insightful research.
Product Name:
Mouse NGF (Nerve growth factor) ELISA Kit
SKU:
MOES01287
Target:
Mouse NGF (Nerve growth factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NGF. You can calculate the concentration of Mouse NGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
86-101
95-107
Average (%)
95
93
102
1:4
Range (%)
90-102
81-95
87-99
Average (%)
96
87
94
1:8
Range (%)
88-100
78-91
83-94
Average (%)
93
85
88
1:16
Range (%)
86-99
79-92
88-101
Average (%)
92
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.56
276.26
909.82
115.72
280.76
929.09
Standard deviation
5.32
16.44
38.67
6.68
15.3
46.55
C V (%)
5.04
5.95
4.25
5.77
5.45
5.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed.
