The Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit is specifically designed for the accurate detection of NGAL levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NGAL is a key protein involved in various biological processes, including inflammation and kidney injury.
It serves as a biomarker for conditions such as acute kidney injury, sepsis, and certain cancers, making it an important target for research and potential therapeutic development.With the Mouse NGAL ELISA Kit, researchers can confidently measure NGAL levels in mouse samples, providing valuable insights into the role of this protein in disease pathogenesis and progression.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NGAL. You can calculate the concentration of Mouse NGAL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
94-107
89-104
Average (%)
97
99
96
1:4
Range (%)
90-104
86-96
83-95
Average (%)
97
91
89
1:8
Range (%)
89-102
79-93
89-104
Average (%)
94
86
95
1:16
Range (%)
87-99
88-99
86-99
Average (%)
92
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
91-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
119.4
390.07
1093.66
121.64
352.2
1131.18
Standard deviation
6.93
17.28
43.64
7.99
18.81
60.41
C V (%)
5.8
4.43
3.99
6.57
5.34
5.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NGAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NGAL in samples. No significant cross-reactivity or interference between Mouse NGAL and analogues was observed.
