The Mouse MUC5AC (Mucin 5 Subtype AC) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MUC5AC levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.MUC5AC is a mucin protein that plays a crucial role in mucin production and secretion, particularly in the respiratory tract. Abnormalities in MUC5AC expression have been associated with various respiratory conditions such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis.
The Mouse MUC5AC CLIA Kit allows for the precise measurement of MUC5AC levels, enabling researchers to study the role of this protein in respiratory diseases and develop potential therapeutic strategies.Overall, the Mouse MUC5AC CLIA Kit is a valuable tool for researchers interested in studying mucin biology and investigating the mechanisms underlying respiratory diseases in mouse models. Its high sensitivity and specificity make it a reliable choice for accurate MUC5AC quantification in experimental settings.
Product Name:
Mouse MUC5AC (Mucin-5 subtype AC) CLIA Kit
SKU:
MOES00441
Target:
Mouse MUC5AC (Mucin-5 subtype AC)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5AC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MUC5AC. You can calculate the concentration of Mouse MUC5AC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
98-112
86-98
Average (%)
98
105
91
1:4
Range (%)
92-109
90-104
97-109
Average (%)
100
97
103
1:8
Range (%)
94-105
98-113
90-106
Average (%)
99
104
97
1:16
Range (%)
90-106
92-104
99-114
Average (%)
98
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.95
252.01
807.62
105.65
272.52
801.24
Standard deviation
9.13
25.71
70.59
13.25
24.64
82.21
C V (%)
8.23
10.2
8.74
12.54
9.04
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MUC5AC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MUC5AC in samples. No significant cross-reactivity or interference between Mouse MUC5AC and analogues was observed.
