The Mouse MMP-8 (Matrix Metalloproteinase-8) ELISA Kit is a highly reliable tool for the quantitative detection of MMP-8 levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for various research applications.Matrix metalloproteinase-8 (MMP-8) is an important enzyme involved in extracellular matrix remodeling, specifically in the degradation of collagen. Its dysregulation has been linked to various pathological conditions, including inflammatory diseases, cancer metastasis, and tissue injury.
The Mouse MMP-8 ELISA Kit provides valuable insights into MMP-8 levels, enabling researchers to better understand its role in disease progression and develop potential therapeutic strategies.Overall, the Mouse MMP-8 ELISA Kit from Assay Genie offers researchers a powerful tool to study MMP-8 dynamics in mouse models, advancing our knowledge of its involvement in various biological processes and diseases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-8. You can calculate the concentration of Mouse MMP-8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
90-107
92-106
Average (%)
96
98
99
1:4
Range (%)
99-114
84-96
96-111
Average (%)
105
90
102
1:8
Range (%)
94-107
82-92
99-114
Average (%)
101
87
104
1:16
Range (%)
100-115
82-97
93-106
Average (%)
108
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
89-103
97
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.5
8.9
1.1
2.7
9.1
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.3
C V (%)
10
4.0
4.49
9.09
3.7
3.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-8 in samples. No significant cross-reactivity or interference between Mouse MMP-8 and analogues was observed.
