The Mouse MMP-8 (Matrix Metalloproteinase-8) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MMP-8 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.Matrix Metalloproteinase-8 is an important enzyme involved in tissue remodeling and inflammation, playing a key role in various physiological and pathological processes.
Its dysregulation is associated with conditions such as arthritis, periodontal diseases, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic targets.
Product Name:
Mouse MMP-8 (Matrix Metalloproteinase 8) CLIA Kit
SKU:
MOES00438
Target:
Mouse MMP-8 (Matrix Metalloproteinase 8)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-8. You can calculate the concentration of Mouse MMP-8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
101-117
103-119
Average (%)
98
108
109
1:4
Range (%)
89-102
93-104
97-114
Average (%)
96
99
105
1:8
Range (%)
94-109
97-112
84-97
Average (%)
100
103
90
1:16
Range (%)
94-109
98-113
96-107
Average (%)
101
105
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.84
529.08
1759.27
214.68
558.78
1698.12
Standard deviation
26.71
61.43
116.82
18.87
59.12
118.36
C V (%)
12.61
11.61
6.64
8.79
10.58
6.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-8 in samples. No significant cross-reactivity or interference between Mouse MMP-8 and analogues was observed.
