The Mouse MMP-3 (Matrix Metalloproteinase-3) ELISA Kit is a specialized assay designed for the quantitative measurement of MMP-3 levels in mouse biological samples including serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and precision, delivering reliable and consistent results for a variety of experimental needs.Matrix Metalloproteinase-3, also known as stromelysin-1, is a key enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a critical role in processes such as wound healing, inflammation, and cancer progression.
By accurately measuring MMP-3 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Mouse MMP-3 ELISA Kit is a valuable tool for studying the role of MMP-3 in various physiological and pathological conditions. Whether investigating arthritis, cardiovascular diseases, or cancer, this kit provides a reliable means of quantifying MMP-3 levels and advancing research in the field of matrix metalloproteinases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-3. You can calculate the concentration of Mouse MMP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
92-108
86-100
Average (%)
94
98
91
1:4
Range (%)
99-114
83-96
95-107
Average (%)
105
88
101
1:8
Range (%)
96-106
87-99
93-105
Average (%)
101
92
98
1:16
Range (%)
96-113
83-94
96-113
Average (%)
104
89
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.2
0.5
1.2
4.6
Standard deviation
0.03
0.06
0.21
0.03
0.06
0.17
C V (%)
6
5.45
5.0
6.0
5.0
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-3 in samples. No significant cross-reactivity or interference between Mouse MMP-3 and analogues was observed.
