The Mouse MMP-2 (Matrix Metalloproteinase 2) CLIA Kit is a powerful tool for the quantitative detection of MMP-2 levels in mouse serum, plasma, and tissue homogenates. This kit offers unparalleled sensitivity and specificity, providing researchers with accurate and reliable results for a variety of experimental applications.MMP-2, a key enzyme in the extracellular matrix, plays a critical role in tissue remodeling, wound healing, and tumor metastasis. Dysregulation of MMP-2 activity has been implicated in various pathological conditions, including cancer, arthritis, and cardiovascular diseases, making it a valuable target for therapeutic interventions and disease monitoring.
With its superior performance and easy-to-use format, the Mouse MMP-2 CLIA Kit is an essential tool for studying MMP-2 biology and potential therapeutic strategies. Trust Assay Genie for high-quality research reagents that deliver exceptional results. Order your kit today and advance your research with confidence.
Product Name:
Mouse MMP-2 (Matrix Metalloproteinase 2) CLIA Kit
SKU:
MOES00434
Target:
Mouse MMP-2 (Matrix Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-2. You can calculate the concentration of Mouse MMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
90-105
94-106
Average (%)
91
97
101
1:4
Range (%)
102-116
99-117
95-107
Average (%)
108
107
100
1:8
Range (%)
102-113
85-98
90-102
Average (%)
107
91
95
1:16
Range (%)
96-112
100-114
96-113
Average (%)
104
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.12
237.77
724.76
102.51
233.33
670.34
Standard deviation
7.88
17.98
70.66
9.52
22.52
64.42
C V (%)
8.2
7.56
9.75
9.29
9.65
9.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-2 in samples. No significant cross-reactivity or interference between Mouse MMP-2 and analogues was observed.
