The Mouse MMP-13 (Matrix Metalloproteinase-13) ELISA Kit is designed for the precise quantification of MMP-13 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MMP-13 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in tissue remodeling and repair processes.
Dysregulation of MMP-13 has been linked to various pathological conditions, including arthritis, cancer, and inflammatory diseases, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.With its advanced technology and ease of use, the Mouse MMP-13 ELISA Kit from AssayGenie is an essential tool for researchers studying MMP-13 biology and its implications in disease pathogenesis.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-13. You can calculate the concentration of Mouse MMP-13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
97-111
92-110
Average (%)
100
103
100
1:4
Range (%)
90-103
82-95
82-93
Average (%)
97
88
87
1:8
Range (%)
88-102
80-90
83-98
Average (%)
94
85
90
1:16
Range (%)
91-103
84-97
86-100
Average (%)
96
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.57
753.47
2413.58
266.68
745.97
2482.18
Standard deviation
17.12
30.97
121.16
18.4
30.96
114.68
C V (%)
6.57
4.11
5.02
6.9
4.15
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-13 in samples. No significant cross-reactivity or interference between Mouse MMP-13 and analogues was observed.
