The Mouse MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is a reliable and accurate tool for measuring MMP-12 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.MMP-12 is a key enzyme involved in tissue remodeling and inflammation, playing a critical role in various physiological and pathological processes. It is implicated in conditions such as chronic obstructive pulmonary disease (COPD), atherosclerosis, and cancer, making it a valuable biomarker for studying and understanding these diseases.
By using the Mouse MMP-12 ELISA Kit, researchers can gain valuable insights into MMP-12 levels in mouse samples, aiding in the development of novel therapeutic strategies and the advancement of scientific knowledge in the field of matrix metalloproteinases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-12. You can calculate the concentration of Mouse MMP-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
90-101
96-114
Average (%)
97
96
104
1:4
Range (%)
94-107
85-97
87-101
Average (%)
99
91
92
1:8
Range (%)
89-104
79-91
89-102
Average (%)
96
85
94
1:16
Range (%)
91-106
85-99
83-97
Average (%)
98
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
0.9
4.61
0.49
0.98
4.86
Standard deviation
0.03
0.04
0.2
0.03
0.06
0.17
C V (%)
5.77
4.44
4.34
6.12
6.12
3.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-12 in samples. No significant cross-reactivity or interference between Mouse MMP-12 and analogues was observed.
