The Mouse MMP-10 (Matrix Metalloproteinase 10) CLIA Kit is specifically designed for the accurate measurement of MMP-10 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.MMP-10 is an important enzyme that plays a key role in tissue remodeling and the degradation of extracellular matrix proteins. It is involved in various physiological processes as well as pathological conditions such as cancer, inflammatory diseases, and fibrosis.
Understanding the activity of MMP-10 can provide valuable insights into disease progression and potential therapeutic targets.With the Mouse MMP-10 CLIA Kit, researchers can easily quantify MMP-10 levels in mouse samples, facilitating the study of its biological functions and implications in different diseases. Trust in the reliability and accuracy of this kit for your research needs.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-10. You can calculate the concentration of Mouse MMP-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
94-108
84-96
Average (%)
94
101
90
1:4
Range (%)
90-101
97-108
91-101
Average (%)
96
103
96
1:8
Range (%)
89-102
100-119
96-107
Average (%)
94
108
102
1:16
Range (%)
100-112
88-99
86-96
Average (%)
106
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
101-114
108
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.44
137.05
448.45
51.32
150.25
463.03
Standard deviation
7.0
16.2
48.39
4.11
13.34
33.89
C V (%)
12.63
11.82
10.79
8.01
8.88
7.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-10 in samples. No significant cross-reactivity or interference between Mouse MMP-10 and analogues was observed.
