The Mouse MMP-1 (Matrix Metalloproteinase-1) ELISA Kit is specially designed for the precise measurement of MMP-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, perfect for various research purposes.MMP-1 is a key enzyme involved in extracellular matrix remodeling, playing a crucial role in tissue repair, inflammation, and cancer progression.
By detecting MMP-1 levels, researchers can gain valuable insights into disease processes and potential therapeutic targets, making this kit an essential tool for investigating various pathological conditions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-1. You can calculate the concentration of Mouse MMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
90-104
92-102
Average (%)
93
97
97
1:4
Range (%)
101-115
80-93
96-111
Average (%)
107
86
103
1:8
Range (%)
95-111
81-92
96-108
Average (%)
101
87
101
1:16
Range (%)
98-110
83-95
99-111
Average (%)
103
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
427.1
740.6
3534.9
385.5
686.0
3417.1
Standard deviation
28.2
38.5
183.8
23.9
30.2
112.8
C V (%)
6.6
5.2
5.2
6.2
4.4
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-1 in samples. No significant cross-reactivity or interference between Mouse MMP-1 and analogues was observed.
