Mouse MIP 3a Recombinant Protein (RPPB1227)
- SKU:
- RPPB1227
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Mouse
- Target:
- MIP 3a
- Synonyms:
- Small inducible cytokine A20 precursor
- CCL20
- Macrophage inflammatory protein 3 alpha
- MIP-3-alpha
- Source:
- Escherichia Coli
- Uniprot:
- O89093
Description
| Product Name: | Mouse MIP 3a Recombinant Protein |
| Product Code: | RPPB1227 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | MIP 3a |
| Synonyms: | Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, C-C motif chemokine 20, CC chemokine ST38, exodus-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized MIP-3a in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized MIP-3a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL-20 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM |
| Biological Activity: | The Activity is calculated by its ability to chemoattract primary human T cells at 2-20ng/ml, corresponding to a specific activity of 50,000-500,000 units/mg. |
CCL20 is a chemotactic factor that draws lymphocytes & neutrophils, rather than monocytes. MIP-3 alpha inhibits proliferation of myeloid progenitors in colony formation assays. MIP3A plays a role in the formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. CCL20 holdes antibacterial activity e.coli atcc 25922 and s.aureus atcc 29213. CCL20 gene transcription is activated by H. pylori, which activates NF-kappaB through intracellular signal pathway which involves IkappaB kinase and NF-kappaB-inducing kinase. MIP-3 alpha is invloved in chemokine-mediated lymphocyte trafficking during gastric inflammation in Helicobacter infection. CCL20 expression is involved in the recruitment of CD45R0-positive T cell subsets into the intestinal lamina propria. MIP-3A is in charge of the advancement of pulpal inflammation through the recruitment of C-C motif Receptor 6-expressing lymphocytes Vaginal epithelial cells respond to factors present in semen by secreting MIP-3 alpha, which increases langerhans cells recruitment during HIV transmission.
Macrophage Inflammatory Protein-3 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.9 kDa. The MIP-3a is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted Cellular Component: extracellular space; extracellular region Molecular Function:protein binding; chemokine activity; cytokine activity Biological Process: immune response; chemotaxis; inflammatory response; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process |
| UniProt Code: | O89093 |
| NCBI GenInfo Identifier: | 6094384 |
| NCBI Gene ID: | 20297 |
| NCBI Accession: | O89093.1 |
| UniProt Secondary Accession: | O89093,Q9Z1X3, |
| UniProt Related Accession: | O89093 |
| Molecular Weight: | 10,755 Da |
| NCBI Full Name: | C-C motif chemokine 20 |
| NCBI Synonym Full Names: | chemokine (C-C motif) ligand 20 |
| NCBI Official Symbol: | Ccl20 |
| NCBI Official Synonym Symbols: | CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1 |
| NCBI Protein Information: | C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine |
| UniProt Protein Name: | C-C motif chemokine 20 |
| UniProt Synonym Protein Names: | Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20 |
| Protein Family: | C-C motif chemokine |
| UniProt Gene Name: | Ccl20 |
| UniProt Entry Name: | CCL20_MOUSE |
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