Mouse MIP-3 beta (Macrophage Inflammatory Protein 3 beta) CLIA Kit
The Mouse MIP-3 Beta (Macrophage Inflammatory Protein 3 Beta) CLIA Kit is a reliable and accurate tool for detecting levels of MIP-3 Beta in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MIP-3 Beta is a key chemokine involved in immune responses, specifically in recruiting and activating immune cells such as macrophages.
It plays a crucial role in the inflammatory process, making it a valuable biomarker for studying inflammatory conditions and potential therapeutic interventions.Overall, the Mouse MIP-3 Beta CLIA Kit is a valuable resource for researchers looking to study the role of MIP-3 Beta in immune responses and inflammatory diseases in mouse models.
Product Name:
Mouse MIP-3 beta (Macrophage Inflammatory Protein 3 beta) CLIA Kit
SKU:
MOES00430
Target:
Mouse MIP-3 beta (Macrophage Inflammatory Protein 3 beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-3β/ELC/CCL19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-3β/ELC/CCL19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-3β/ELC/CCL19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIP-3β/ELC/CCL19. You can calculate the concentration of Mouse MIP-3β/ELC/CCL19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
85-97
86-97
Average (%)
109
91
91
1:4
Range (%)
90-103
86-101
92-105
Average (%)
97
93
99
1:8
Range (%)
85-96
88-101
95-109
Average (%)
91
93
103
1:16
Range (%)
99-115
87-99
99-115
Average (%)
105
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.33
44.61
209.41
24.9
41.81
206.14
Standard deviation
3.18
3.98
18.13
2.46
4.12
16.33
C V (%)
11.64
8.92
8.66
9.88
9.85
7.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MIP-3β/ELC/CCL19 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIP-3β/ELC/CCL19 in samples. No significant cross-reactivity or interference between Mouse MIP-3β/ELC/CCL19 and analogues was observed.
