Mouse MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
The Mouse MIP-3 Alpha (Macrophage Inflammatory Protein-3 Alpha) CLIA Kit is specifically designed for the quantification of MIP-3 Alpha levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MIP-3 Alpha is a chemokine that plays a crucial role in the recruitment of immune cells to sites of inflammation and infection. It is involved in the regulation of immune responses and can be a key player in various inflammatory disorders and autoimmune diseases.
Understanding the levels of MIP-3 Alpha in biological samples can provide valuable insights into the immune response and aid in the development of targeted therapies for these conditions.Overall, the Mouse MIP-3 Alpha CLIA Kit offers researchers a reliable tool for studying the role of MIP-3 Alpha in immune regulation and inflammatory processes in mouse models.
Product Name:
Mouse MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
SKU:
MOES00012
Target:
Mouse MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-3α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-3α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIP-3α. You can calculate the concentration of Mouse MIP-3α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
90-104
86-101
Average (%)
91
96
93
1:4
Range (%)
89-104
93-107
86-99
Average (%)
96
99
91
1:8
Range (%)
89-104
92-105
100-116
Average (%)
95
100
108
1:16
Range (%)
93-106
92-105
84-95
Average (%)
100
99
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
96-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.14
63.01
198.68
19.28
61.77
196.14
Standard deviation
2.1
4.71
21.5
1.55
6.6
20.71
C V (%)
10.43
7.48
10.82
8.04
10.68
10.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MIP-3α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIP-3α in samples. No significant cross-reactivity or interference between Mouse MIP-3α and analogues was observed.
